Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: International Journal of Dentistry

Article Title: The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

doi: 10.1155/2024/3746794

Figure Lengend Snippet: Culture and characterization of hDPSCs: (a) hDPSCs were isolated from the coronal pulp of human third molars via enzymatic digestion and tissue cultivation; (b) vimentin protein was positive in hDPSCs (immunohistochemistry staining); (c) CD73, CD90, STRO-1, and CD45 were analyzed by flow cytometry; and (d) hDPSCs were cultured in osteogenic or adipogenic induction medium. Scale bar: 20 μ m. hDPSC, human dental pulp stem cell.

Article Snippet: For adipogenic differentiation, we use the hDPSCs Adipogenic Differentiation Medium Kit (HUXDP-90031, Cyagen, Suzhou, China).

Techniques: Isolation, Immunohistochemistry, Staining, Flow Cytometry, Cell Culture

Journal: International Journal of Dentistry

Article Title: The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

doi: 10.1155/2024/3746794

Figure Lengend Snippet: Differentiation of hDPSCs into glial cells: (a) schematic diagram of the culture assay. hDPSCs cultured in β -ME medium for ∼1 day before further seeding in RA culture medium for 3 days, followed by culture in Forskolin, b-FGF, PDGFaa, and NRG-1 medium. Cells and cell supernatant were collected for further characterization; (b) immunofluorescent staining was performed on hDPSCs and SC-DPSCs for the typical Schwann cell markers MBP, p75NTR, and S-100. Scale bar: 20 μ m. hDPSC, human dental pulp stem cell; PDGFaa, platelet-derived growth factor AA; SC, stem cell; (c) RT-PCR analysis of the expression of Schwann cell marker MBP, p75NTR, and S-100. b-FGF, basic fibroblast growth factor; PDGFaa, platelet-derived growth factor AA; NRG-1, neuregulin-1; MBP, myelin basic protein; p75NTR, p75 neurotrophin receptor; and β -ME, β -mercaptoethanol. ∗ P < 0.05.

Article Snippet: For adipogenic differentiation, we use the hDPSCs Adipogenic Differentiation Medium Kit (HUXDP-90031, Cyagen, Suzhou, China).

Techniques: Cell Culture, Staining, Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Marker

Journal: International Journal of Dentistry

Article Title: The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

doi: 10.1155/2024/3746794

Figure Lengend Snippet: Neurotrophin expression in hDPSCs after induction: (a) enzyme-linked immunosorbent assays indicated a significant increase and dynamic tendency in BDNF and NT-3 levels after differentiation; (b) the expression of genes coding for BDNF and NT-3 in hDPSCs was analyzed by qRT-PCR; and (c) immunofluorescent staining was performed to detect NT-3 expression. hDPSC, human dental pulp stem cell; BDNF, brain-derived neurotrophic factor; and NT-3, neurotrophin-3. ∗ P < 0.05.

Article Snippet: For adipogenic differentiation, we use the hDPSCs Adipogenic Differentiation Medium Kit (HUXDP-90031, Cyagen, Suzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Staining, Derivative Assay

Journal: PLoS ONE

Article Title: Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

doi: 10.1371/journal.pone.0164918

Figure Lengend Snippet: (A) Microscope images of undifferentiated ASCs at passage 3; Alizarin Red-stained osteogenic ASCs and Oil-O-Red-stained adipocytes differentiated into ASCs. Scale bar = 50 μm. (B) Flow cytometric analysis of ASCs. The ASCs were analyzed by flow cytometry with fluorochrome-conjugated monoclonal antibodies against CD90, CD73s, and CD45.

Article Snippet: Briefly, for adipogenic differentiation, the cultured cells were induced with ASCs adipogenic differentiation medium kit (Cyagen Biosciences, China).

Techniques: Microscopy, Staining, Flow Cytometry, Bioprocessing

Journal: PLoS ONE

Article Title: Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

doi: 10.1371/journal.pone.0164918

Figure Lengend Snippet: ASCs proliferation (OD at 490 nm) after E2 treatment at different concentrations from 1 to 8 days. Student’s t-tests were used to evaluate the significance ( p < 0.05) in the OD values of cells treated with different concentrations of E2 with those of the control groups (E2-free)(*) and the 10 −9 M E2 group (#).

Article Snippet: Briefly, for adipogenic differentiation, the cultured cells were induced with ASCs adipogenic differentiation medium kit (Cyagen Biosciences, China).

Techniques: Control

Journal: PLoS ONE

Article Title: Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

doi: 10.1371/journal.pone.0164918

Figure Lengend Snippet: (A) Phase-contrast images of MD-ASCs treated with and without E2 supplementation for 2 and 4 weeks. Scale bar = 100 μm. (B) Cells were immunostained with antibodies directed against α-SMA (red) and MHC (green); the nuclei are counterstained with DAPI (blue). Scale bar = 50 μm.

Article Snippet: Briefly, for adipogenic differentiation, the cultured cells were induced with ASCs adipogenic differentiation medium kit (Cyagen Biosciences, China).

Techniques:

Journal: PLoS ONE

Article Title: Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

doi: 10.1371/journal.pone.0164918

Figure Lengend Snippet: (A) Relative expression of myogenic differentiation markers (relative to GAPDH) was measured by quantitative RT–PCR during the myogenic differentiation of ASCs with or without exposure to E2 for 2 or 4 weeks. (B) Western blotting results of the myogenic differentiation of ASCs with or without exposure to E2 for 2 or 4 weeks.

Article Snippet: Briefly, for adipogenic differentiation, the cultured cells were induced with ASCs adipogenic differentiation medium kit (Cyagen Biosciences, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: PLoS ONE

Article Title: Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

doi: 10.1371/journal.pone.0164918

Figure Lengend Snippet: (A) Scanning electron microscopy (SEM) of the morphology of MD-ASCs after 24 h of incubation on the nano-scaffold. (a and b) MD-ASCs treated without E2. (c and d) MD-ASCs treated with E2. Scale bar = 50 μm. (B) Cellular proliferation on a nano-scaffold with or without E2 supplementation. Student’s t -tests were used to compare the OD values between groups with and without E2 supplementation (* indicates significance p < 0.05).

Article Snippet: Briefly, for adipogenic differentiation, the cultured cells were induced with ASCs adipogenic differentiation medium kit (Cyagen Biosciences, China).

Techniques: Electron Microscopy, Incubation